牛冷冻精液不同有效精子数输精效果的研究

Study on the Effect of Sperm Transfusion with Different Effective Sperm Counts in Frozen Semen of Cattle

中文关键词: 种公牛  冷冻精液  有效精子数  输精  受胎率

英文关键词: breeding bull  frozen semen  effective sperm count  Insemination  Conception rate

中文摘要:

      [目的] 肉牛种业为肉牛生产发展的基础和前提,种公牛是肉牛业发展的“芯片”,直接关系到生产的质量和效率。为提高优秀种公牛的利用率和区域覆盖率,适当降低每剂量冷冻精液的有效精子数量,同时能够确保其输精受胎率不受影响而进行该研究。[方法]制作含有效精子数分别为8ｘ10₆^、4ｘ10₆^、2ｘ10₆^,三组牛冷冻精液,用上述冷冻精液进行输精试验,比较输精效果。[结果] 有效精子数为8ｘ10₆^、有效精子数为4ｘ10₆^、有效精子数为2ｘ10₆^,三个试验组间其人工输精后受胎率进行了比较。研究结果表明,输精总受胎率差异不显著,降低有效精子数,其输精受胎率未受影响。[结论]降低每剂冷冻精液的有效精子数量(2~8ｘ10₆⁾在适当范围内,其输精受胎率差异不显著。

英文摘要:

      [Objective] Beef cattle seed industry is the foundation and premise of beef cattle production and development, and breeding bull is the "chip" of beef cattle industry development, which is directly related to the efficiency and quality of production. In order to improve the utilization rate and coverage of excellent breeding bulls, the effective sperm number per dose of frozen semen was appropriately reduced, and the fertility rate of insemination was not affected. [Method] Three groups of frozen semen containing effective sperm were prepared with 8x10₆^{, 4x10}₆ ^{and 2x10₆} sperm, and the insemination test was carried out with the frozen semen mentioned above, and the insemination effect was compared. [Result] The fertility rate after artificial insemination was compared between the three experimental groups of 8x10₆^{, 4x10}₆ ^{and 2x10₆}. The results showed that there was no significant difference in the total fertility rate of the shaft, and the fertility rate of sperm was not affected by the reduction of the effective sperm count. [Result] The effective sperm count per dose of frozen semen (2~8ｘ10^₆) was reduced, and the fertilization rate was not significantly different.