肉牛口蹄疫免疫抗体、3ABC抗体、RT-PCR核酸检测结果分析

Analysis of FMD Antibody,3ABC Antibody and RT-PCR Nucleic Acid Detection Results in Beef Cattle

中文关键词: 肉牛  口蹄疫  免疫抗体  3ABC抗体  RT-PCR核酸检测

英文关键词: beef cattle  foot and mouth disease  immune antibody  3ABC antibody  RT-PCR nucleic acid detection

中文摘要:

      [目的]为了掌握肉牛口蹄疫免疫抗体检测结果、口蹄疫非结构蛋白3ABC抗体检测结果、口蹄疫O-P液RT-PCR核酸检测结果三者的相关性，[方法]张掖市动物疫病预防控制中心对甘州区28头肉牛开展相关检测。[结果]检测结果表明：O型口蹄疫免疫抗体合格率28份，合格率100%，3ABC抗体阳性27份，阳性率96.4%；对3ABC抗体阳性的27头牛分2次进行实时荧光RT-PCR核酸检测，被检的27头牛均为阴性。

英文摘要:

      [Objective] In order to master the correlation among the detection results of foot and mouth disease (FMD) immune antibody, FMD nonstructural protein 3ABC antibody and FMD O-P solution RT-PCR nucleic acid, [Method] Zhangye animal disease prevention and control center carried out the relevant detection on 28 beef cattle in Ganzhou district. [Result] The results showed that the qualified rate of O-FMD antibody was 28, the qualified rate was 100%, the positive rate of 3ABC antibody was 27, the positive rate was 96.4%; 27 cattle with 3ABC antibody positive were detected by real-time RT-PCR twice, and all 27 cattle were negative.