通辽地区犊牛咳喘病主要病原血清抗体检测

Detection of serum antibodies against the main pathogens of calf cough and asthma disease in Tongliao area

中文关键词: 犊牛  咳喘  血清流行病学  ELISA检测

英文关键词: Calves  Coughing and wheezing  Sero-epidemiology  ELISA

中文摘要:

      [目的]为了解通辽市犊牛咳喘病主要病原牛传染性鼻气管炎病毒（Bovine rotavirus，BRV）牛病毒性腹泻病毒（BVDV）、牛副流感病毒3型（BPIV3）、多杀性巴氏杆菌（Pasteurella multocida，Pm）的血清流行情况。[方法]使用抗体检测试剂盒，对7个地区的213家养殖场（户）肉牛场采集的400份血清样品进行ELISA抗体检测。[结果]显示7个旗县区IBRV、BVDV、BPIV3和Pm的样品抗体阳性率分别为26.50%、19.25%、2.50%、33.50%；不同地区的IBRV、BVDV、BPIV3和Pm抗体阳性率分别为8.33%～41.79%、10.26%～32.26%、0%～6.12%和17.95%～46.77%；IBRV抗体阳性检出率在秋、冬两季最高，分别为32.22%和30.08%，BVDV抗体阳性检出率在春、冬两季最高，分别为19.72%和22.56%，BPIV3抗体阳性检出率在冬季，为3.01%，Pm抗体阳性检出率在秋、冬两季，阳性率分别为36.67%和39.85%；养殖场（户）存在IBRV、BVDV、Pm多种病原混合感染，主要以二重感染中IBRV+Pm为主，阳性率为7.75%，存在三重感染，尚未发现四重感染。结果提示应加强对上述病原流行的控制，提高犊牛饲养管理。本研究为犊牛咳喘病相关病原菌防控提供一定的参考数据。

英文摘要:

      [Purpose] To understand the serum prevalence of Bovine Rotavirus (BRV), Bovine Viral Diarrhea Virus (BVDV), Bovine Parainfluenza Virus 3 (BPIV3), and Pasteurella Multicida (Pm), the main pathogens of cough and asthma in calves in Tongliao City. [Method] ELISA antibody detection was performed on 400 serum samples collected from 213 beef farms (households) in 7 regions using antibody detection kits. [Results] The antibody positivity rates of IBRV, BVDV, BPIV3, and Pm samples in 7 counties and districts were 26.50%, 19.25%, 2.50%, and 33.50%, respectively; The positive rates of IBRV, BVDV, BPIV3, and Pm antibodies in different regions were 8.33% to 41.79%, 10.26% to 32.26%, 0% to 6.12%, and 17.95% to 46.77%, respectively; The positive detection rates of IBRV antibodies were highest in autumn and winter, at 32.22% and 30.08%, respectively. The positive detection rates of BVDV antibodies were highest in spring and winter, at 19.72% and 22.56%, respectively. The positive detection rates of BPIV3 antibodies were 3.01% in winter, and the positive detection rates of Pm antibodies were 36.67% and 39.85% in autumn and winter, respectively; There is a mixed infection of IBRV, BVDV, and Pm in the breeding farm (household), mainly consisting of IBRV+Pm in the secondary infection, with a positive rate of 7.75%. There is a triple infection, and no quadruple infection has been detected yet. The results suggest that it is necessary to strengthen the control of the epidemic of the above-mentioned pathogens and improve the management of calf feeding. This study provides certain reference data for the prevention and control of pathogenic bacteria related to calf cough and asthma disease.