腐熟牛粪中耐高温高效纤维素分解菌的筛选及其复合菌系的降解效果

Screening of Thermotolerant and High-Efficiency Cellulose-Decomposing Bacteria from Composted Cattle Manure and Degradation Efficiency of Composite Bacterial Consortia

中文关键词: 纤维素分解菌  菌株筛选  牛粪  降解效果

英文关键词: cellulose decomposing bacterium  strain screening  cattle manure activity  degradation efficiency

中文摘要:

      采用纤维素钠平板法和刚果红染色法，从自然发酵腐熟的牛粪中分离得到高效纤维素分解菌，通过16S rDNA分子生物学技术对菌种进行鉴定。以羧甲基纤维素酶活性为筛选指标，基于滤纸酶活性测试和拮抗试验构建复合菌系，并评价其对金针菇菌棒的降解效果。结果表明，筛选获得2株具有较高纤维素酶活性的菌株，鉴定分别属于台湾假黄单胞菌属（Pseudoxanthomonas taiwanensis）和芽孢杆菌属（Bacillus）。2株菌等比例复配得到复合菌系，其产羧甲基纤维素酶活性为25.22U/mL，滤纸酶活性为14.95U/mL。复合菌系对金针菇菌棒表现出较好的降解能力，降解率高达47.47%。

英文摘要:

      In this study, high-efficiency cellulose decomposing bacterium were isolated from naturally fermented and decomposed cattle manure by cellulose sodium plate method and Congo red staining method, and the strains were identified by 16S rDNA molecular biology technology. Carboxymethyl cellulase activity was used as the screening index, and the compositing strain was constructed based on the filter paper enzyme activity test and antagonistic experiment, and its degradation effect on the stick of enoki mushroom was evaluated. The results showed that two strains with high cellulase activity were identified as Pseudoxanthomonas taiwanensis andBacillus. The compound strains were compounded in equal proportions by the two strains, and the carboxymethyl cellulase activity was 25. 22 U/mL, and the filter paper enzyme activity was 14. 95 U/mL. The composite strains showed good degradation ability to Enoki mushroom sticks, with a degradation rate of 47. 47%.