家驴MRFs基因家族全基因组鉴定及转录组分析

Genome wide Identification and Transcriptome Analysis of the MRFs Gene Family in Domestic Donkeys

中文关键词: 家驴  MRFs基因家族  生物信息学  转录组分析

英文关键词: domestic donkey  MRFs gene family  bioinformatics  transcriptome analysis

中文摘要:

      MRFs基因家族是肌肉发生过程中参与分子调控的一个重要的基因家族，对脊椎动物肌细胞的分化和骨骼肌系统的发育成熟具有重要意义。研究旨在对家驴MRFs基因家族进行全面系统的鉴定分析，研究MRFs基因在家驴不同组织中的表达模式，明确家驴MRFs基因家族的组成、功能和进化关系。研究运用HMMER并结合BLAST对家驴的MRFs基因家族成员进行鉴定，通过多种生物信息学方法对其蛋白序列进行分析，运用转录组数据分析MRFs基因家族在家驴不同组织中的表达规律，并对MRFs基因家族进行GO功能富集分析。结果显示，家驴MRFs基因家族共有4个成员，均为亲水性蛋白；其家族成员蛋白二级结构以无规则卷曲为主，α-螺旋次之，β-折叠最少；系统进化树显示，家驴、人和小鼠3个物种的MRFs家族基因基本呈现出相间排列的规律，有较近的进化关系；基因结构分析表明，MRFs基因家族成员具有较高的相似性；蛋白保守基序分析表明，其具有较高的保守性；转录组数据显示，MyoG在肌肉组织中高表达。GO功能富集分析发现，MRFs基因家族参与调控肌肉生成的全过程。

英文摘要:

      The MRFs gene family plays a crucial role in molecular regulation during myogenesis and holds significant importance in myocyte differentiation and skeletal muscle system development in vertebrates.This study aimed to comprehensively identify and analyze the MRFs gene family in domestic donkeys,investigate its expression patterns across various tissues,and elucidate the composition,functional characteristics,and evolutionary relationships of this gene family.The research employed HMMER and BLAST for systematic identification of MRFs family members,followed by multi faceted bioinformatics analyses of protein sequences.Transcriptome data were utilized to examine tissue-specific expression profiles,and functional enrichment analysis was conducted.Results demonstrated that the domestic donkey MRFs gene family comprises four members encoding hydrophilic proteins,with secondary structures predominantly composed of random coils,followed by alpha-helices and minimal beta-sheets.Phylogenetic analysis revealed close evolutionary relationships among domestic donkey,human,and mouse MRFs genes,exhibiting interspersed clustering patterns.Gene structure analysis indicated high similarity among family members,supported by conserved protein motifs.Transcriptome profiling identified MyoG as highly expressed in muscle tissues.Functional enrichment analysis confirmed the MRFs family’s involvement in regulating the entire myogenic process.