黄牛 HAVCR2基因拷贝数变异与生长性状的关系

Genome-wideIdentification and Transcrip to me Analysis o ftheMRFs Gene Family in Domestic Donkeys

中文关键词: 黄牛  HAVCR2基因  拷贝数变异  生物信息学分析

英文关键词: HAVCR2 gene  copy number variation  bioinformatics analysis

中文摘要:

      DNA拷贝数变异已被公认为遗传变异的重要来源,本研究利用 生物信息学相关网 站分析了 HAVCR2基因的理化特性,采用实时荧光定量 PCR 对中国 4 个黄牛群体共 795 头牛 HAVCR2基因的拷贝数变异类型进行检测,并比较了拷贝数变异类型与 4 个黄牛群体 体重和体尺性状之间的关系。 结果显示,HAVCR2为稳定疏水蛋白,分子质量为 30925. 6 ku, 理论等电点为 6. 52,含有信号肽,具有 3 个糖基化位点, 与 CD4、CD80、IFNG、TIGIT等蛋 白相互作用。 HAVCR2基因多拷贝变异型对郏县红牛体高有极显著( P<0. 01)影响,对郏 县红牛十字部高和尻长 、对皮南牛体斜长均有显著( P<0. 05)影响,表明 HAVCR2基因的 拷贝变异对中国 肉牛的生长发育有一定影响。

英文摘要:

      DNA copy number variation (CNV) has been recognized as an important source of genetic variation. In this study , we analyzed the physical and chemical properties of HAVCR2 gene by using bioin- format ics-related websites. Real time fluorogenic quantitative PCR was used to detect copy number varia- tion types of HAVCR2 gene in 795 cattle from 4 cattle populations in China, and the relationship between copy number variation types and body weight and body size traits in four cattle populations was also com- pared. The results showed that HAVCR2 was a s table hydrophobic protein with a molecular mass of 30 925. 6 ku and a theoretical isoelectric point of 6. 52. HAVCR2 contained signal peptides and had three g lycosylation sites. The multi-copy variation of HAVCR2 gene had a significant effect on Jiaxian Red cattle’s body height (P<0. 01) , cross height and length of Jiaxian Red cattle, and body length of Pinan cattle( p<0. 05) , indicating that the copy variation of HAVCR2 gene had a certain effect on the growth and de- velopment of Chinese cattle.