新疆昌吉州肉牛血液原虫病流行情况及防治措施

Epidemic situation and prevention and control measures of blood protozoa in beef cattle in Changji Prefecture, Xinjiang

中文关键词: 肉牛  宏基因  肉牛血液原虫

英文关键词: Beef cattle  Metagene  Beef cattle blood parasites

中文摘要:

      为调查新疆昌吉州肉牛主要血液原虫病原，本试验采集病牛抗凝血282份，眼鼻拭子14份、组织样品8份、粪便样品12份等共计34份，利用mNGS宏基因组测序技术和酶联免疫吸附试验（ELISA）抗体检测方法开展牛血液原虫检测，结果发现主要检出病原为巴贝斯虫属（Babesia bigemina）和泰勒虫属（Theileria ）两类，其中牛环形泰勒虫病检测270份次，阳性检出率51.5%；牛双芽巴贝斯虫检测184份次，阳性检出率65.6%；牛巴贝斯虫在辖区未检出。并对14份抗凝血样品进行PCR验证，结果一致。据检测结果制定以三氮脒和咪唑苯脲进行药物预防和治疗，效果显著。本研究为昌吉州区制定有效的肉牛血液原虫综合防治措施提供有效基础数据。

英文摘要:

      To investigate the primary blood protozoal pathogens affecting beef cattle in Changji Prefecture, Xinjiang, this study collected a total of 282 anticoagulant blood samples from sick cattle, along with 14 eye and nose swabs, 8 tissue samples, and 12 fecal samples, utilizing mNGS metagenomic sequencing technology and the Enzyme-linked immunosorbent assay (ELISA) for protozoa detection in cattle blood. The results indicated that the predominant pathogens identified were Babesia bigemina and Theileria. Specifically, the detection of bovine annular theileriosis yielded a positive detection rate of 51.5% (270 cases), while Babesia bovis had a positive detection rate of 65.6% (184 cases); notably, Babesia bovis was not detected within the jurisdiction. PCR verification was conducted on 14 anticoagulated blood samples, yielding consistent results. Based on these findings, a drug prevention and treatment regimen involving triazamidine and imidazole was implemented, demonstrating significant efficacy. This study provides valuable foundational data for the formulation of effective comprehensive prevention and control measures against blood protozoa in beef cattle within Changji Prefecture.