利用基因组重测序技术鉴定彭阳县夏洛莱牛的纯度

Assessment of Genetic Purity in Charolai cattle in Pengyang County Using Whole Genome Re-Sequencing

中文关键词: 夏洛莱牛  全基因组重测序  遗传结构  纯度

英文关键词: Charolais cattle, whole-genome resequencing, genetic structure, purity

中文摘要:

      ［目的］对宁夏彭阳县10头夏洛莱牛进行全基因组重测序，并与下载的48头牛的全基因组重测序数据进行比对分析，以鉴定彭阳县10头夏洛莱牛的纯度。［方法］利用全基因组重测序技术和生物信息学分析方法。［结果］通过对彭阳县10头夏洛莱牛全基因组数据进行分析，共检测到17,583,444个SNP位点，其基因组的核苷酸多样性（0.0016）稍微高于欧洲普通牛（0.0012）和东亚普通牛（0.0011）。遗传结构分析表明，当K=3时，10头彭阳县夏洛莱牛主要为欧洲普通牛血统（64.10% ~ 95.54%），其次是东亚普通牛血统（0.00% ~ 32.25%）和中国瘤牛血统（3.09% ~ 13.51%），说明彭阳县10头夏洛莱牛都存在不同程度的东亚普通牛和中国瘤牛血统。［结论］基于全基因组重测序分析，彭阳县10头夏洛莱牛均为杂交牛。

英文摘要:

      [Objective] To determine the genetic purity of 10 Charolai cattle in Pengyang County, Ningxia, whole genome resequencing was performed and compared with the whole genome resequencing data of 48 cattle downloaded. [Methods] The study utilized whole genome resequencing technology and bioinformatics analysis methods. [Results] A total of 17,583,444 SNP loci were identified through whole-genome analysis of 10 Charolai cattle in Pengyang County. The nucleotide diversity of 10 Charolai cattle genome (0.0016) was found to be a little higher to that of European common cattle (0.0012) and East Asian common cattle (0.0011). The genetic structure analysis showed that when K=3, the 10 Chalolai cattle were mainly European taurine cattle ancestry (64.10% ~ 95.54%), followed by East Asian taurine cattle ancestry (0.00% ~ 32.25%) and Chinese Zebu cattle ancestry (3.09% ~ 13.51%). The results indicated that 10 Charolai cattle in Pengyang County had different degrees of East Asian taurine cattle and Chinese Zebu cattle ancestry. [Conclusion] The whole genome resequencing analysis indicates that all 10 Charolai cattle in Pengyang County are hybrid cattle.