哈密市牛环形泰勒虫病诊断与治疗

Diagnosis and control of theileriosis annularis in Hami cattle

中文关键词: 哈密市  牛  环形泰勒虫  诊断  防治

英文关键词: Hami city  cattle  theileria annulata  diagnosis  control

中文摘要:

      为了探讨环形泰勒虫病诊断与防治效果,试验采用临床诊断、病理剖检、血液涂片染色镜检、PCR检测及硬蜱形态学鉴定等方法,针对2023年5月哈密市部分养殖户的牛出现发热、淋巴结肿大等症状,病死率达43.6 %,为尽快控制病情,采集病牛血样60份,分别进行血液涂片检测和PCR诊断。结果表明：血液涂片的阳性率为55 %；PCR检测阳性率为65 %,进行测序比对分析发现,病原为环形泰勒虫,两种检测方法的阳性符合率为81.8 %,其中养殖场的发病率高于散养户,改良牛的感染率明显高于本地牛,以2岁以下牛发病较为多见。对病死牛剖检肉眼可见,可视黏膜苍白或黄染、心脏外膜出血点、肝脏淤血、肿大,有暗红色病灶等病理变化。采集患牛体表寄生硬蜱,通过形态学鉴定确定为小亚璃眼蜱。说明针对不同病程,使用不同的处方治疗,牛环形泰勒虫病可得以控制。现提出诊断、治疗及预防措施,以期为环形泰勒虫病的防控提供参考。

英文摘要:

      In order to explore the diagnosis and control effects of Bovine tropical theileriosis, test employed various methods including clinical diagnosis, pathological autopsy, blood smear, PCR detection, and morphological identification of hard ticks.aim at May 2023 cattle from some farmers in Hami City showed clinical symptoms such as fever and swollen lymph nodes, with a mortality rate of 43.6%, to control the disease as soon as possible, Blood samples were taken from 60 cattle, the examination of blood smear and PCR were performed.The results indicated that,showing a positive rate of 55% and 65%, respectively. Sequencing and comparative analysis revealed that the pathogen was Theileria annulata, and the positive coincidence rate of the two detection methods was 81.8%. Farms had a higher morbidity rate than sporadic breeding, and improved cattle had a significantly higher infection rate than local cattle. The disease was more common in cattle under 2 years old. Autopsy of diseased cattle revealed characteristic signs such as visible mucosal pallor or yellowing, bleeding points on the epicardium, liver congestion, swelling, and dark red lesions. The hard ticks parasitizing on the surface of diseased cattle were collected and morphologically identified as Hyalomma anatolicum anatolicum. Different prescriptions were used according to different courses of the disease, theileriosis was controlled. The diagnosis, treatment, and prevention suggestions are reported below to provide a reference for the prevention and control of tropical theileriosis.