饲喂水牛不同部位脂肪组织对金黄地鼠健康的影响

The effect of feeding different parts of adipose tissue of buffalo on the health of golden hamsters

中文关键词: 水牛  胸部皮下脂  肾周脂  不饱和脂肪酸

英文关键词: Bubalus Bubalis  sternum subcutaneous adipose tissue  perirenal adipose tissue  unsaturated fatty acids

中文摘要:

      本研究旨在探究饲喂水牛胸部皮下脂（不饱和脂肪酸含量高）和肾周脂（饱和脂肪酸含量高）对金黄地鼠健康的影响。本试验以成年摩拉水牛胸部皮下脂肪和肾周脂肪组织为材料，将30只6周龄雌性金黄地鼠随机平均分为3组：普通饲喂组（含油量10%）、胸部皮下脂高脂饲喂组（含油量45%）、肾周脂高脂饲喂组（含脂量45%）。适应性饲喂1周，正式饲喂试验为期8周。在第0、4、8周采集血液样本，饲喂结束时采集肝脏和腹股沟脂肪组织，试验期间每周称重，每天称量采食量。结果表明：与普通饲喂组相比，肾周脂高脂饲喂8周金黄地鼠终末体重、平均日增重、Lee’s指数、血脂浓度（甘油三酯、胆固醇等）、体脂沉积、肝脏损伤及脂代谢等均显著升高（p<0.05或p<0.01），提示高脂饲喂可引起金黄地鼠肥胖；同样是高脂饲喂，胸部皮下脂饲喂组金黄地鼠终末体重、平均日增重、Lee’s指数、血脂浓度（胆固醇、动脉硬化指数等）、体脂沉积、肝脏损伤及脂代谢等均显著均低于肾周脂饲喂组（p<0.05或p<0.01）。综上所述，富含不饱和脂肪酸的水牛胸部皮下脂可缓解高脂饲喂带来的健康风险，这为水牛不饱和脂肪酸生成的调控提供重要基础信息和材料。

英文摘要:

      The aim of this study is to investigate the effects of feeding buffalo sternum subcutaneous adipose tissue (SSAT, with high unsaturated fatty acids content) and perirenal adipose tissue (PRAT, with high saturated fatty acids content) on the health of golden hamsters. The SSAT and PRAT of Mora buffalo (24-month-old) were used. A total of 30 golden hamsters (female, 6-week-old) were randomly divided into 3 groups on average, namely normal feed group (ND group, 10% oil content), SSAT high-fat feeding group (HFD-SSAT group, 45% oil content), and PRAT high-fat feeding group (HFD-PRAT group, 45% oil content). Adaptive feeding for 1 week and official feeding for 8 weeks. Blood samples were collected in weeks 0, 4, and 8. Liver and inguinal adipose tissue were collected at the end of feeding. During the experiment, body weight was measured weekly and food intake was measured daily. As results, compared to the ND group, the final body weight, average daily weight gain, Lee""s index, blood lipid concentration (triglycerides, cholesterol, etc.), body fat deposition, liver injury, and lipid metabolism of golden hamsters in HFD-PRAT group were significantly increased (p<0.05 or p<0.01), indicating that high-fat feeding can cause obesity in golden hamsters. Under the high-fat feeding, the final body weight, average daily gain, Lee""s index, blood lipid concentration (cholesterol, arteriosclerosis index, etc.), body fat deposition, liver injury, and lipid metabolism of golden hamsters in HFD-SSAT group were significantly lower than those in HFD-PRAT group (p<0.05 or p<0.01). In summary, buffalo SSAT, which is rich in unsaturated fatty acids, can alleviate the health risks brought by high-fat feeding. These results provide important basic information and materials for the regulation of unsaturated fatty acid production in buffalo.