利用基因组重测序技术鉴定黑安格斯牛的纯度

Assessment of Genetic Purity and population genomic features in Black Angus Cattle Using Whole Genome Re-Sequencing

中文关键词: 黑安格斯牛  遗传多样性  群体遗传结构  纯度  全基因组

英文关键词: Black Angus cattle  genetic diversity  population genetic structure  purity  whole genome

中文摘要:

      【目的】基于10头黑安格斯牛和60头对照组牛的全基因组重测序数据，分析黑安格斯牛的纯度、遗传多样性及群体结构。【方法】全基因组重测序技术和生物信息学方法。【结果】通过对10头黑安格斯牛全基因组数据进行分析，共检测到15,064,459个SNP位点，其核苷酸多样性（pi）为0.0015，观测杂合度（Ho）为0.2381，期望杂合度（He）为0.2430，表明黑安格斯牛的遗传多样性较低；主成分分析和群体遗传结构分析发现，黑安格斯牛与欧洲普通牛聚为一类，其中有4头黑安格斯牛存在偏离情况，表明这4头牛主要与中国瘤牛与东亚普通牛韩牛之间存在杂交。【结论】10头黑安格斯牛中，6头为纯种黑安格斯牛，4头为杂种牛。

英文摘要:

      [Objective] This study utilizes whole-genome resequencing technology to evaluate the genetic integrity and diversity of Black Angus cattle. [Methods] Employing whole-genome resequencing data from 10 Black Angus cattle and 60 control group cattle using bioinformatics tools and analyses, this study investigates the genetic purity, diversity, and population structure. [Results] The whole-genome sequence data analysis of 10 Black Angus cattle revealed 15,064,459 SNP sites. The nucleotide diversity (pi) was recorded at 0.0015, with observed heterozygosity (Ho) at 0.2381 and expected heterozygosity (He) at 0.2430, indicating a low genetic diversity within the breed. Principal component analysis and population genetic structure assessments showed that Black Angus cattle generally cluster with European taurine cattle. Notably, deviations in four Black Angus cattle suggest hybridization events predominantly with Chinese indicine cattle and East Asian breeds, such as Korean cattle. [Conclusion] Among the cattle examined, six were identified as purebred Black Angus, while four exhibited signs of hybridization.