叉头转录因子FoxO1的生物遗传信息学分析

Biogenetic Informatics Analysis of Forkhead Transcription Factor FoxO1Yong Liu1, Wenke Zhu3, Xinyi Wu2,3, Mengmeng Lu2,3, Yujia Sun2,3

中文关键词: 牛，FoxO1基因，遗传结构，生物化学信息

英文关键词: Cattle, FoxO1, genetic  structure,biochemical  information

中文摘要:

      叉头转录因子FoxO1是一种重要的调控因子，其广泛参与机体的各项生命活动，对其生物信息学的分析，可以更清楚和直观的了解FoxO1基因的结构和功能，为后期发育、分化、代谢和进化等作用机制的深入研究提供重要的理论基础。本试验运用生物信息学手段对牛FoxO1基因的结构和功能进行预测分析，结果表明FoxO1蛋白为不稳定的亲水性酸性蛋白质，主要定位在细胞核，含有七个半胱氨酸残基，其中六个半胱氨酸形成三个二硫键，3个氨基酸 具有59个潜在磷酸化位点，作为非分泌型蛋白，无信号肽和跨膜区域，其二级结构中，无规则卷曲所占比例高达75.42%，且在第165-259位氨基酸之间，其叉头域有95个保守的氨基酸残基。

英文摘要:

      The forked transcription factor FoxO1 is an important regulatory factor that widely participates in various life activities. Analyzing FoxO1 bioinformatics could provide a clearer and more intuitive understanding of the structure and function of the FoxO1 gene, providing an important theoretical basis for in-depth research on the mechanisms of later development, differentiation, metabolism, and evolution. We also used bioinformatics methods to carry on the forecast analysis of the structure and function of FoxO1 protein ,such as physicochemical properties, hydrophobicity, phosphorylation sites, transmembrane domain, subcellular localization, signal peptide, disulfide bond, secondary structure and conserved domain .It turned out that, FoxO1 protein is the unstable acidic and the hydrophilic protein ,having phosphorylation site, and no transmembrane regions; FoxO1 protein mainly located in nucleus, secondly in the cytoplasm, and having no signal peptide; it is non secretory protein, and there are seven cysteine residues, including six cysteine form three disulfide bond; In the secondary structure, the proportion of random curl is as high as 75.42%, alpha helix take second place at 17.30%, and Forkhead domain has 95 conservative amino acid residues between amino acids 165- 259.