患病和痊愈牦牛源大肠杆菌对氨基糖苷类和磺胺类耐药性比较分析

Comparative analysis of antibacterial resistance to aminoglycosides and sulfonamides of Escherichia coli isolated from sick and recovered yaks

中文关键词: 牦牛源大肠杆菌  氨基糖苷类  磺胺类  药物敏感性试验  耐药基因

英文关键词: Yak-derived Escherichia coli  Aminoglycosides  Sulphonamides  Antibacterial sensitivity test  Antibacterial resistance genes

中文摘要:

      [目的]为了探讨患病和痊愈牦牛源大肠杆菌的抗菌药物及其耐药性之间的相关性。[方法]采用微量肉汤稀释法对91株牦牛源大肠杆菌进行氨基糖苷类药物（链霉素和庆大霉素）和磺胺类药物（磺胺甲噁唑和磺胺二甲基嘧啶）敏感性试验，同时采用PCR方法检测氨基糖苷类相关耐药基因 aac(6')-Ib、aac(3')-IV及磺胺类相关耐药基因sul2、sul3的携带情况。[结果]结果表明：39株患病牦牛源菌株对链霉素、庆大霉素、磺胺甲噁唑和磺胺二甲基嘧啶的耐药率分别为30.77%、5.13%、61.54%和87.18%，aac(6')-Ib、aac(3')-IV、sul2和sul3的检出率分别为43.59%、25.64%、38.46%和41.03%；52株痊愈牦牛源菌株对链霉素、庆大霉素、磺胺甲噁唑和磺胺二甲基嘧啶的耐药率分别为42.30%、21.15%、94.23%和71.15%，aac(6')-Ib、aac(3')-IV、sul2和sul3的检出率分别为71.15%、19.23%、69.23%和51.92%。[结论]试验结果表明，痊愈牦牛源大肠杆菌对氨基糖苷类和磺胺类药物耐药率及相关耐药基因检出率均高于患病动物源大肠杆菌，应科学合理选用和使用抗菌药物以减少耐药性的产生以提升牦牛大肠杆菌病的治疗效果。

英文摘要:

      [Objective] To investigate the relationship between antibacterial drugs and antibacterial resistance of Escherichia coli isolated from sick and recovered yaks, [Method] In this study, micro-broth dilution method was used for antibacterial sensitivity test of 91 yak-derived Escherichia coli to aminoglycosides (Streptomycin and Gentamicin) and sulphonamides (Sulphamethoxazole and Sulfamethazine) and aminoglycoside-related resistance genes aac(6')-Ib, aac(3')-IV and sulfonamide-related resistance genes sul2 and sul3 were detected by PCR method. [Result] The results showed that the antibacterial resistance rates of 39 Escherichia coli isolated from sick yaks to Streptomycin, Gentamicin, Sulphamethoxazole and Sulfamethazine were 30.77%、5.13%、61.54% and 87.18%, respectively, and the detection rates of antibacterial resistance genes of aac(6')-Ib, aac(3')-IV, sul2 and sul3 were 43.59%、25.64%、38.46% and 41.02%, respectively; The antibacterial resistance rates of 52 Escherichia coli isolated from recovered yaks to Streptomycin, Gentamicin, Sulphamethoxazole and Sulfamethazine were 42.30%、21.15%、94.23% and 71.15%, respectively, and the detection rates of antibacterial resistance genes of aac(6')-Ib, aac(3')-IV, sul2 and sul3 were 71.15%、19.23%、69.23% and 51.92%, respectively. [Conclusion] The experimental results revealed that both antibacterial resistance rate and detection rate of antibacterial resistance genes of Escherichia coli isolated from recovered yaks were higher than sick yaks, and rational select and correct use of antibacterial drugs should be scientifically to reduce the occurence of drug resistance and improve the therapeutic effect of colibacillosis in yaks.