河西走廊地区犊牛腹泻致病性大肠杆菌分离鉴定、毒力因子及耐药性检测

Isolation, identification virulence factors and drug resistance test of pathogenic E.coli from calf diarrhea in Hexi Corridor

中文关键词: 犊牛  腹泻  大肠杆菌  致病性  毒力基因  耐药性  

英文关键词: calf  Diarrhea  E.coli  Pathogenicity  Virulence gene  Drug resistance  

中文摘要:

      为了分析河西走廊地区犊牛腹泻致病性大肠杆菌携带毒力基因和耐药性情况,2020年~2021年在河西走廊地区采集患腹泻病犊牛的粪便、肛拭子及肝脏等病料组织279份,采用常规的方法和PCR方法进行大肠杆菌分离鉴定。采用人工感染试验动物、PCR方法和K-B药敏纸片法对分别检测犊牛腹泻性大肠杆菌致病性、毒力因子和耐药性。结果表明,分离得到了126株大肠杆菌, 其中79株犊牛腹泻性大肠杆菌能引起小鼠死亡；分离的79株致病性大肠杆菌的毒力基因crl 、irp2、fimH、papC、K88、K99、stx1、stx2检测率在40.5%~100%之间,其他毒力基因检测率在15.2%~34.2%之间；分离的79株致病性大肠杆菌对氨苄西林、阿莫西林、新霉素等8种药物耐药率在49.4%~96.2%之间,对其他药物的耐药率在5.1%~32.9%之间。结论：从河西走廊地区患腹泻病犊牛病料组织中分离得79株致病性大肠杆菌,79株致病性大肠杆菌携带多种毒力基因,对临床中常用的抗菌药物产生了耐药性。本研究为河西走廊地区犊牛腹泻致病性大肠杆菌的防治奠定了基础。

英文摘要:

      In order to analyze the virulence gene and drug resistance of calf diarrhea pathogenic E.coli in Hexi Corridor, From 2020 to 2021, 279 diseased tissues such as feces, anal swabs and liver of calves suffering from diarrhea disease were collected in Hexi Corridor, and coliform bacteria were isolated and identified by conventional methods and PCR methods. The pathogenicity, virulence factors and drug resistance of calf diarrhea E.coli were detected by artificial infection test animals, PCR method and K-B drug sensitive paper method. The results showed that 126 strains of E.coli were isolated, among which 79 strains of calf diarrhea E.coli could cause death of mice; The detection rates of virulence genescrl,irp2,fimH,papC,K88,K99,stx1 and stx2 of 79 strains of pathogenic Escherichia coli were 40.5%~100%, and the detection rates of other virulence genes were 15.2%~34.2%; The resistance rate of 79 strains of pathogenic E.coli isolated to 8 drugs, such as ampicillin, amoxicillin and neomycin, was 49.4%~96.2%, and the resistance rate to other drugs was 5.1%~32.9%. Conclusion: 79 strains of pathogenic E. coli were isolated from the diseased tissues of calves suffering from diarrhea in the Hexi Corridor. 79 strains of pathogenic E.coli carry multiple virulence genes and are resistant to commonly used antibiotics in clinical practice. This study laid a foundation for the prevention and treatment of pathogenic E.coli in calf diarrhea in Hexi Corridor.