牛4种主要传染病的检测与分析

Detection and analysis of four major infectious diseases in cattle

中文关键词: 口蹄疫  布鲁氏菌病  结核病  牛结节性皮肤病  检测  分析

英文关键词: Foot-and-Mouth Disease  Brucellosis  Bovine Tuberculosis  Lumpy Skin Disease  detection  analysis

中文摘要:

      [目的]旨在研究口蹄疫、布鲁氏菌病、牛结核病、牛结节性皮肤病等4种牛主要传染病的免疫效果和流行情况。[方法]采用疫苗对口蹄疫、牛结节性皮肤病免疫，针对性使用荧光PCR、ELISA方法以及虎红平板凝集试验、皮内变态反应试验对上述4种牛病进行检测。[结果]免疫牛口蹄疫10.21万头次，检测血清1 534份和组织样品233份，口蹄疫免疫抗体合格率94.33%，口蹄疫病毒为阴性。使用山羊痘疫苗免疫牛结节性皮肤病2.89万头次，检测口鼻拭子/抗凝样品200份，牛结节性皮肤病病毒为阴性。监测牛布鲁氏菌病血清2380份，阳性率为0.17%。监测牛结核病1 291头，阳性率为0.23%。[结论]口蹄疫、牛结节性皮肤病免疫效果良好，达到阻断病毒感染和传播目的。布鲁氏菌病、牛结核病总体上分别达到控制标准和净化标准，但“两病”仍有零星发生，需要加强牛只调运监管检疫。

英文摘要:

      [Objective]The purpose is to investigate the immune effect and prevalence of four major infectious diseases of cattle, including Foot-and-mouth disease（FMD）, brucellosis, bovine tuberculosis（bTB）and Lumpy Skin Disease（LSD）.[Method]The vaccine was used to immunize against foot-and-mouth disease and Lumpy Skin Disease.Fluorescent PCR, ELISA,Rose Bengal Test and PPD intradermal allergy test were used to detect the above four bovine diseases.[Result]A total of 102 100 cattle were immunized against FMD.1534 serum samples and 233 tissue samples were tested.The qualified rate of FMD antibody was 94.33%, and the FMD virus was negative. A total of 28,900 cattle were immunized with goat pox vaccine against LSD, and 200 oral and nasal swabs and anticoagulant samples were tested, which were negative for LSDV. 2380 serum samples of bovine brucellosis were monitored, and the positive rate was 0.17%. 1291 cattle were monitored for tuberculosis, and the positive rate was 0.23%. [Conclusion]The results showed that the immune effect of foot-and-mouth disease and Lumpy Skin Disease was good, and the purpose of blocking virus infection and transmission was achieved. On the whole, brucellosis and bovine tuberculosis meet the control and purification standards respectively, but the diseases still occur sporadically, and it is necessary to strengthen the supervision and quarantine of cattle transportation.