沃金黑牛GPR41基因多态性及其与不同阶段生长性状的相关性分析

Correlation between polymorphism in exon 2 of GPR41 gene and growth traits of Wojin Black cattle

中文关键词: 沃金黑牛  GPR41基因  单核苷酸多态性  单倍型  生长性状

英文关键词: Wojin Black cattle  GPR41 gene  Single nucleotide polymorphism  Haplotype  growth traits  

中文摘要:

      为探究GPR41基因多态性对沃金黑牛不同阶段生长性状的影响，选取63头沃金黑牛（去势公牛）为研究对象，采用Sanger测序技术检测单核苷酸多态性（SNP）位点，利用Haploview软件进行单倍型分析。利用“牛等家养动物群体遗传分析工具V1.0”评价沃金黑牛群体遗传结构，利用SPSS 19.0软件分析GPR41基因多态性与生长性状的相关性。结果显示：沃金黑牛GPR41基因外显子2存在2个突变位点，分别为S1：Chr18:46058902bp处的G/T突变和S2：Chr18:46058908bp处的C/T突变；S1、S2位点多态性与沃金黑牛不同生长阶段部分生长性状存在显著相关（P＜0.05）；2个SNPs位点共形成3种有效单倍型组合，单倍型组合H2H2（TT/CC）显著影响沃金黑牛体斜长与背膘厚。

英文摘要:

      In order to research the polymorphism of GPR41 among different growth stages Wojin black cattle, selected 63 Wojin black cattle(castrated bull) as research subjects. PCR product Sanger sequencing were used to detect SNP sites, haplotype analysis were analyzed by Haploview. Genetic structure was evaluated by "cattle and other domestic animal population genetic analysis tool V1.0", the correlation between gene polymorphism and growth traits were analyzed by SPSS19.0. Results showed: there were two mutation sites in GPR41 exon 2, such as S1:Chr18:46058902bp G/T and S2:Chr18:46058908bp C/T ; Polymorphism of S1 and S2 had significant effects on some growth traits at Wojin black cattle different growth stage (P＜0.05); There were three effective haplotype combinations at two mutation sites,H2H2(TT/CC) significantly affected chest circumference and backfat thickness of Wojin black cattle.