中国荷斯坦牛RPL23A和ACACB基因对产奶性状的遗传效应分析

Genetic Effect Analysis of RPL23A and ACACB Genes for Milk Production in Chinese Holstein Cattle

中文关键词: 产奶性状  RPL23A基因  ACACB基因  SNP  遗传效应  中国荷斯坦牛

英文关键词: Milk production traits  RPL23A gene  ACACB gene  SNP  Genetic effect  Chinese Holstein cattle

中文摘要:

      作者所在团队前期通过奶牛乳腺上皮组织转录组测序及荷斯坦公牛全基因组重测序研究发现RPL23A和ACACB基因是奶牛乳蛋白和乳脂性状的候选功能基因,本研究旨在探究这两个基因是否对奶牛产奶性状具有显著遗传效应。以北京地区7个牧场的1059头中国荷斯坦母牛为试验群体,采集尾根静脉血并提取基因组DNA,通过飞行时间质谱方法检测SNP位点基因型,利用SAS9.4软件的MIXED过程进行关联分析。结果表明,RPL23A基因的SNP位点g.20146771C>T与第1泌乳期5个产奶性状达到显著或极显著关联(P=0.0001~0.0416),其优势等位基因为T；ACACB基因的g.63878254T>C位点与第1泌乳期产奶量、乳脂量和乳蛋白量呈极显著关联(P<0.01),其优势等位基因为C；g.63962768G>A位点与第1泌乳期产奶量、乳脂量、乳脂率和乳蛋白率关联显著或极显著(P=0.0001~0.0391),其优势等位基因为A。综上,RPL23A基因主要影响中国荷斯坦牛产奶量和乳蛋白,ACACB基因对产奶量和乳脂具有显著遗传效应,3个SNP位点可考虑作为遗传标记用于标记辅助选择培育奶牛高乳蛋白乳脂新品系和选育提高。

英文摘要:

      Previously, author"s team found that RPL23A and ACACB genes were closely related to milk production traits of Chinese Holstein cattle by transcription of cow mammary epithelial tissue and genome re-sequencing. In order to study the effect of these 2 genes on milk yield, milk fat content, milk protein content, milk protein content and milk protein content of Holstein cows, molecular markers related to milk production traits were found. DNA was extracted from 1059 Chinese Holstein cows from 7 cattle farmsSin Beijing and detection of SNP genotypes by MALDI-TOF. MIXED process in SAS9.4 software was used to correlate SNP loci with 5 milk-producing traits. Correlation analysis showed that SNP locus g.20146771C>T of RPL23A gene was significant or extremely significant in 5 milk production traits (P=0.0001~0.0416) during lactation stage 1, and its dominant allele T. In the first lactation stage, ACACB gene locus g.63878254T>C was extremely significantly correlated with milk yield, fat yield and protein yield (P<0.01), and its dominant allele C; and g.63962768G>A was significantly or extremely significantly correlated with milk yield, fat yield, fat percentage and protein yield (P=0.0001~0.0391), and its dominant allele A. In conclusion, the RPL23A gene mainly affects milk yield and milk protein in Chinese Holstein cattle, ACACB gene has a significant effect on milk yield and milk fat inheritance, and 3 SNP loci may be considered as genetic markers for marker assisted selection for breeding new lines of high milk protein and high milk fat, and improve the breeding.