单细胞测序技术及其在畜牧科学研究中的应用

Single Cell Sequencing Technology and Its Application in Livestock Science Research

中文关键词: 单细胞测序  异质性  畜牧科学

英文关键词: single cell Sequencing  heterogeneity  livestock science

中文摘要:

      单细胞测序(Single Cell Sequencing)是对单个细胞进行测序后利用生物信息学等手段进行分析的技术,包括单细胞分离、扩增测序和数据分析3个方面,主要应用于肿瘤、胚胎发育、免疫学等方面。随着单细胞测序技术的迅速发展,单细胞测序技术已成为研究细胞发育中基因组、转录组和表观基因组异质性的重要工具。单细胞测序可以获取单个细胞完整的全基因组,克服了大样本量、细胞异质性等问题,为研究细胞间异质性信息和细胞群体之间关系提供了新的研究策略。本文就单细胞测序方法及其在畜牧科学研究中的应用进行综述,并探讨其应用前景。

英文摘要:

      Single Cell Sequencing is essentially the analysis of a Single Cell using bioinformatics and other technologies after Sequencing. It is mainly used in tumor, embryo development, immunology and other aspects. With the rapid development of single-cell sequencing technology, single-cell sequencing technology has become an important tool to study the heterogeneity of genome, transcriptome and epigenome during cell development. Single cell sequencing, by obtaining the complete genome of a single cell, overcomes the problems of large sample size and cell heterogeneity, and provides a new technology for studying the information of inter-cell heterogeneity and the relationship between cell populations. The paper reviewed the sequencing methods of single cell sequencing and its application in livestock science.