蒙古牛线粒体DNA全基因组遗传多样性与起源研究

The Genetic Diversity of Mitochondrial DNA Genome of Mongolian Cattle

中文关键词: 蒙古牛  mtDNA  遗传多样性  母系起源

英文关键词: Mongolian Cattle  mtDNA  genetic diversity  origin

中文摘要:

      ［目的］通过对蒙古牛线粒体DNA(mtDNA)的基因组进行测序,探究蒙古牛的mtDNA基因组遗传多样性与母系起源。［方法］采用DNA提取、三代测序及生物信息学方法。［结果］在36头蒙古牛mtDNA全基因组序列中,共检测到22种不同的单倍型,平均单倍型多样度(Hd)为0.970,平均核苷酸多样度(Pi)为0.00845,表明蒙古牛有丰富的母系遗传多样性。构建的IQ系统发育树发现,蒙古牛具有瘤牛和普通牛两个母系支系。［结论］蒙古牛有丰富的母系遗传多样性,拥有普通牛和瘤牛两个母系起源,以普通牛起源为主。

英文摘要:

      [Objective] To explore the genetic diversity and maternal origin of Mongolian Cattle. [Method] Using whole genome sequencing and Bioinformatics methods. [Result] 22 mtDNA haplotypes were defined. The average haplotype diversity (Hd) is 0.970, and the average nucleotide diversity (Pi) is 0.00845, indicating that Mongolian Cattle have relatively rich genetic diversity. The constructed IQ phylogenetic tree shows that Mongolian Cattle have two maternal origins: taurine and indicus cattle. [Conclusion] Mongolian Cattle have rich genetic diversity. The phylogenetic analysis showed that Mongolian Cattle had two female branches, taurine and indicine.