不同稀释液及稀释比例对关中奶山羊精液低温保存效果的影响

Effects of Different Diluents and Diluting Ratios on the Low-temperature Preservation of Guanzhong Dairy Goat Semen

中文关键词: 关中奶山羊精液  4℃保存  精液稀释液  稀释比例  精子活率  精子有效保存时间

英文关键词: Guanzhong dairy goat semen  Storage at 4°C  Semen dilution  Dilution ratio  Sperm motility  Effective sperm survival time

中文摘要:

      【目的】本试验通过比较精子活率和精子的有效存活时间，探讨在4℃环境下不同稀释液对关中奶山羊精子保存效果的影响，并使用保存效果最好的稀释液进一步研究关中奶山羊精液最佳稀释比例。【方法】试验共设计了6种精液稀释液配方，对精液进行1:4稀释，每间隔6h检测精子活率并记录精子有效存活时间，筛选出最佳稀释液配方；使用筛选出的稀释液配方对精液进行不同比例的稀释（1:2、1:4、1:8、1:16和1:32），每间隔6h检测精子活率并记录精子有效存活时间，筛选出最佳稀释比例。【结果】以柠檬酸钠作为主要缓冲物质并添加15%卵黄的C组稀释液，在相同保存时间下的精子活率和精子的有效存活时间均显著优于其他5组（P<0.05）；使用C组稀释液对精液进行不同比例稀释后，稀释比例为1:8时在相同保存时间下的精子活率和精子的有效存活时间均显著优于其他组（P<0.05）。【结论】以柠檬酸钠作为主要缓冲物质并添加15%卵黄，稀释比例为1:8时，精液冷冻保存效果最好。

英文摘要:

      【Objective】By comparing the sperm viability and the effective survival time of sperm, this experiment discussed the effects of different diluents on the sperm preservation effect of Guanzhong Dairy Goat at 4 ℃, and further studied the best dilution ratio of Guanzhong dairy goat semen by using the diluent with the best preservation effect. 【Methods】Six kinds of semen diluent formulations were designed. The semen was diluted 1:4. The sperm viability was detected every 6 hours and the effective survival time of sperm was recorded. The best diluent formulation was selected; the semen was diluted in different proportions with the selected diluent formulation (1:2, 1:4, 1:8, 1:16 And 1:32). The sperm viability and effective survival time were measured every 6 hours, and the best dilution ratio was selected.【Results】The sperm viability and effective survival time of group C with sodium citrate as the main buffer and 15% yolk were significantly better than those of the other 5 groups at the same storage time (P<0.05); after diluting semen in different proportions with group C diluent, when the dilution ratio is 1:8, the sperm viability and effective survival time under the same preservation time are significantly better than those in other groups (P<0.05).【Conclusion】When sodium citrate is used as the main buffer and 15% egg yolk is added, the semen cryopreservation effect is the best when the dilution ratio is 1:8.