: [Objective] Determine the significance of different methods of brucellosis detection in clinical application. [Method] RBT, SAT and Celisa were used to detect the suspected infected brucellosis sheep serum, and the Kappa value, coincidence rate, sensitivity and specificity were analyzed. [Conclusion]Data showed that the three factors had high coincidence, the Kappa value was above 0.76, and the consistency was quite reliable. The sensitivity of RBT was high, but the false positive rate was also high. Compared with the results of SAT, the sensitivity and specificity of Celisa were both high, and the Kappa value of RBT and CELISA was 0.85, in the comprehensive judgment clinical work, CELISA is more suitable for diagnosis. |