牛病毒性腹泻的鉴定以及治疗

Identification and treatment of bovine viral diarrhea

中文关键词: 牛病毒性腹泻  血清学检测  PCR鉴定

英文关键词: bovine viral diarrhea  Serological test  PCR identification

中文摘要:

      为了鉴定甘肃武威地区某牛场中牛病毒性腹泻病的发病情况，分别采集了13只病牛的血液样品、粪便样品配合诊断，通过血清学诊断方法和PCR鉴定的方法对样本进行了检测，结果显示血清学方法中的13份血液样本有8份为阳性，粪便样本提取RNA，进行RT-PCR同样可以检测到8份样本中扩增得到片段大小为267bp的条带，检测的13份样本有8份样本的血清学检测和粪便PCR检测均为阳性，本次检测的阳性率为61.54%，结果表明本次检测的甘肃武威地区某养殖场的牛病毒性腹泻阳性率相对较高，需要加强牛病毒性腹泻的防控，采取科学的手段治理该类疾病降低其对养牛产业的损失。

英文摘要:

      in order to identify the incidence of bovine viral diarrhea in a cattle farm in Wuwei area of Gansu Province, the blood and fecal samples of 13 sick cattle were collected for diagnosis. The samples were detected by serological diagnosis and PCR identification. The results showed that 8 of 13 blood samples were positive, and RNA was extracted from fecal samples, RT-PCR could also detect the 267bp band in 8 samples. Of the 13 samples tested, 8 samples were positive in serological test and fecal PCR test. The positive rate of this test was 61.54%. The results showed that the positive rate of bovine viral diarrhea in a farm in Wuwei area of Gansu Province was relatively high, It is necessary to strengthen the prevention and control of bovine viral diarrhea, take scientific measures to control the disease, and reduce the loss of cattle industry.