云南省牛病毒性腹泻-黏膜病血清流行病学调查

Seroepidemiological Investigation of Bovine Viral Diarrhea-mucosal Disease in Yunnan Province

中文关键词: 云南省  BVD  血清学调查  抗体、抗原检测

英文关键词: Yunnan province  BVD  serological investigation  antibody and antigen detection

中文摘要:

      [目的]牛病毒性腹泻-黏膜病(bovine viral diarrhea-mucosal disease,BVD)引起牛腹泻、呼吸系统疾病、生殖功能障碍、免疫抑制、母畜流产、死胎等,对牛产业发展危害严重。牛病毒性腹泻-黏膜病(bovine viral diarrhea-mucosal disease virus,BVDV)在我国大部分牛群中普遍存在,各地流行情况严重程度不同,本调查旨在调查云南省BVDV 的流行情况。[方法]调查通过采用商品化的酶联免疫吸附试验(ELISA)试剂盒对BVDV感染和流行情况进行调查,采集了云南省4个州市10个县（市）的部分规模牛场及散养户的牛血清样品456份。[结果]结果发现,云南省不同地区所检的牛血清样品总抗体阳性率为16.45%(75/456),抗原阳性率为0.53%(2/378),其中抗体阳性率以大理州洱源县60.0%(9/15)最高,而丽江市华坪县的血清样品中未检测到抗体阳性,大理州宾川县和丽江市宁蒗县的抗原阳性率较低,分别为7.69%(1/13)和4.00%(1/25)。云南省规模场牛的血清样品抗体总阳性率为27.27%(45/165),抗原阳性率为1.15%(1/87),散养户牛的分别为10.31%(30/291)和0.34%(1/291)。[结论]云南省的牛群中存在着BVDV感染的情况,其抗体抗原的阳性率水平跟地区有着紧密的联系,不同的养殖方式抗原抗体水平存在着一定差异,必须要加强云南省BVDV监测与防控,减少其造成的经济损失。

英文摘要:

      [Objective] Bovine viral diarrhea-mucosal disease (BVD) can cause bovine diarrhea, respiratory disease, reproductive dysfunction, immunosuppression, abortion, and stillbirth for female animals, harmful for cattle industry development. Bovine viral diarrhea-mucosal disease (BVDV) is widespread for many cattle populations in our country, of which the prevalence is different in the different regions. The objective of this survey was to investigate the prevalence of BVDV infection in some areas of Yunnan province. [Methods] It was used a commercial ELISA kit to show the investigation of the infection and prevalence of BVDV. A total of 456 bovine serum samples from some large-scale farms and free-range households in 10 counties/cities from 4 prefectures/cities in Yunnan province were collected in this survey. [Result] The results showed that the antibody detection and antigen detection of bovine serum samples from the different regions of Yunnan province, showing that the total antibody positive rate was 16.45% (75/456), and the antigen positive rate was 0.53% (2/378). The highest antibody positive rate was 60.0%, happened in Eryuan county of Dali prefecture (9/15). No antibody positive rate was detected in Huaping county of Lijiang city. The total antigen positive rates were lower for Binchuan county of Dali prefecture and Ninglang county of Lijiang city, of which the corresponding values were 7.69% (1/13) and 4.0% (1/25). After analyzing the different feeding systems, the positive rate of large-scale farms was 27.27% (45/165) and the positive rate of antigen was 1.15% (1/87), while the positive rate of free-range households was 10.31% (30/291) and the positive rate of antigen is 0.34% (1/291). [Conclusion] There existed BVDV infection for cattle herds in Yunnan, and the positive rate of antibody antigen is closely related to the different regions, and that there are certain differences in antigen and antibody levels in the different feeding systems. Therefore, the prevention and control of BVDV in Yunnan must be strengthened to reduce the economic losses.