云南水牛mtDNA D-loop遗传多样性研究

Genetic Diversity of mtDNA D-loop Region in Yunnan Buffaloes

中文关键词: 云南水牛  mtDNA D-loop  遗传多样性

英文关键词: Yunnan buffalo  mtDNA D-loop  genetic diversity

中文摘要:

      [目的] 探究云南3个水牛品种(德宏水牛、滇东南水牛、盐津水牛)的mtDNA D-loop区的遗传多样性与母系起源。[方法]采用PCR扩增、测序及生物信息学方法。[结果] 本研究共分析了215条云南水牛mtDNA D-loop全序列,检测到107个多态位点,定义了86种单倍型。结果表明,云南3个水牛品种的mtDNA遗传多样性非常丰富,其中,滇东南水牛的遗传多样性最高(Hd: 0.946±0.017,Pi: 0.0162±0.0018),盐津水牛的遗传多样性最低(Hd: 0.805±0.063,Pi: 0.0141±0.0031)。系统发育分析结果表明,在3个云南水牛品种中检测到2个主要支系(A和B支系及其亚支系)及一个稀有支系C,其中A支系为主要支系(79.07%),且经历了强烈的群体扩张。[结论] 云南3个水牛品种具有丰富的mtDNA遗传多样性,主要有A与B两个母系起源。

英文摘要:

      [Objective] The aim of the study was to explore the genetic diversity of three Yunnan buffalo breeds. [Method] The complete mitochondrial DNA (mtDNA) D-loop regions of 215 buffaloes were analyzed using PCR sequencing and bioinformatics methods. [Result] A total of 107 polymorphic sites were detected, which defined 86 haplotypes. Among the three buffalo breeds, the Diandongnan buffalo breed showed the highest genetic diversity (Hd: 0.946±0.017, Pi: 0.01620±0.00179). Our results showed that two main lineages (A and B with their sub-lineages) and one rare lineage (C) were detected in Yunnan buffaloes. The lineage A was extremely frequent in Yunnan buffaloes, and experienced a strong population expansion. [Conclusion] The genetic diversity of these three Yunnan buffalo breeds were abundant, and mainly originated from two lineage A and B of swamp buffalo.