广西的尼里—拉菲水牛和摩拉水牛mtDNA D-loop区遗传多样性研究

Genetic Diversity of mtDNA D-loop Region of Nili-Ravi and Murrah Buffaloes in Guangxi Province

中文关键词: 尼里-拉菲水牛  摩拉水牛  mtDNA D-loop  遗传多样性

英文关键词: Nili-Ravi buffalo  Murrah buffalo  mtDNA D-loop  genetic diversity

中文摘要:

      [目的]探究引入广西南宁的尼里-拉菲水牛和摩拉水牛的mtDNA D-loop区遗传多样性与母系起源。[方法]采用PCR扩增、测序及生物信息学方法。[结果]对从广西南宁采集的52个江河型水牛（尼里-拉菲水牛25个，摩拉水牛27个）mtDNA D-loop序列与GenBank下载的20条尼里-拉菲水牛和23条摩拉水牛序列进行联合分析，共检测到112个变异位点，定义42个单倍型，发现摩拉水牛（Hd: 0.934±0.027）与尼里-拉菲水牛的遗传多样性（Hd: 0.929±0.017）都很丰富。NJ系统发育树显示尼里-拉菲水牛和摩拉水牛含有江河型和沼泽型水牛mtDNA支系，表明尼里-拉菲水牛和摩拉水牛引入中国后均与沼泽型水牛进行了杂交，在外貌上很难区分。[结论]引入广西南宁的尼里-拉菲水牛和摩拉水牛与沼泽型水牛存在广泛的血缘混杂现象。

英文摘要:

      [Objective] To analyze the genetic diversity and maternal origin of the introduced Nili-Ravi and Murrah buffalo in Nanning city, Guangxi Province. [Method] PCR, sequencing and Bioinformatics were used. [Result] The complete mtDNA D-loop regions of 52 introduced river buffaloes (including 25 Nili-Ravi and 27 Murrah buffaloes) in Nanning city, Guangxi Province and 20 Nili-Ravi and 23 Murrah buffaloes downloaded from GenBank were analyzed, a total of 112 polymorphic sites were detected, which constituted 42 haplotypes. The Murrah buffalo and Nili-Ravi buffaloes showed rich genetic diversity with Hd=0.934±0.027 and Hd=0.929±0.017, respectively. NJ tree showed that the Nili-Ravi and Murrah buffalo included the mtDNA lineages of river buffalo and swamp buffalo, which demonstrated that the introduced Nili-Ravi and Murrah buffalo in China crossbred with native swamp buffaloes. Ii is very difficult to distinguish their appearance of Nili-Ravi and Murrah buffalo and their hybrids with swamp buffaloes. [Conclusion] The introduced Nili-Ravi and Murrah buffaloes in Nanning city of Guangxi Province showed extensive introgression with swamp buffaloes.