肉牛支原体病原分离鉴定及药物敏感性试验

Isolation And Identification of Mycoplasma From Beef Cattle And Drug Sensitivity Test

中文关键词: 牛支原体  分离  鉴定  药物

英文关键词: mycoplasma  bovis, separationS,authenticate,medicine

中文摘要:

      [目的]为调查贵州省肉牛运输综合征主要病因牛支原体，研究无菌采集疑似牛支原体鼻拭子和病死牛肺，进行牛支原体液体培养和固体培养，[方法]采用形态学观察和生化试验鉴定该分离株，进行了组织病理切片观察，用特异性检测牛支原体的PCR方法进行检测，并对阳性样本进行药敏试验。[结果]结果显示，从采集到的163份疑似样本中，检测出60份牛支原体阳性样本，且成功分离到18株牛支原体，PCR检测和同源性结果表明扩增片段为牛支原体的特异性条带，同源性的比对结果为93.02%～100%。分离株菌落呈典型的“煎蛋样”。组织切片结果可见患病牛肺间质动脉血管充血、肺间质水肿变宽，呈灰白色；肺间质纤维增生，有实质性病变；肺泡上皮细胞变性坏死；肺泡内有大量粘液。因并发或继发其他病原菌感染，其他器官组织也发生了病理变化。分离株牛支原体既不分解葡萄糖、甘露醇、尿素酶、明胶，也不水解精氨酸，酚红葡萄糖肉汤呈阳性反应，七叶苷生化反应为阳性。药敏试验表明：氟苯尼考、诺氟沙星、新霉素、头孢派酮、氧氟沙星高度敏感。[结论]研究结果证实了引起贵州省肉牛运输综合征主要病因是牛支原体，这为贵州省牛支原体病的防治提供了科学依据。

英文摘要:

      Mycoplasma bovine disease is caused by mycoplasma bovine. It is one of the diseases harmful to cattle industry.In recent years, the introduction of beef cattle in our province has been increasing, which has brought about a series of epidemic diseases of cattle, including infection, occurrence and beef cattle transport syndrome, etc., among which mycoplasma is the main cause of cattle transport syndrome.Since 2016, we have investigated the epidemic situation of the disease, and studied the pathogen isolation and identification, biological characteristics, molecular diagnosis and drug sensitivity, so as to provide technical support for the prevention and control of this disease in our province in the future.