[Objective] To study the difference of semen quality of local cattle breeds in Anhui province, [Method] this experiment analyzed and compared the raw and frozen semen collected from three kinds of cattle in winter and spring, namely Wandong cattle, Dabieshan cattle and Wannan cattle. [Result] The results showed that the ejaculation quantity of Dabieshan cattle was significantly lower than that of Wandong cattle and Wannan cattle (P< 0.05). The semen of all three breeds was milky white. In terms of sperm motility, the sperm motility of Wannan cattle was the highest, followed by that of Wandong cattle, and that of Dabieshan cattle was the lowest. There was no significant difference between different breeds (P> 0.05). After thawing, the activity rate of Wandong cattle was the lowest, which was significantly different from Dabieshan cattle and Wannan cattle (P< 0.05). After thawing, the number of sperm moving in a straight line was the highest in Wannan cattle, which was significantly different from that in Wandong cattle and Dabieshan cattle (P< 0.05). In terms of the number of bacteria in semen, Dabieshan cattle had the highest number of bacteria in each dose of frozen semen, which was significantly different from Wandong cattle and Wannan cattle (P< 0.05). In terms of malformation rate, the sperm malformation rate of Wandong cattle was the lowest, which was significantly different from Dabieshan cattle and Wannan cattle (P< 0.05). [Conclusion] Among the three kinds of bulls, Wandong cattle and Wannan cattle had high ejaculation quantity, low sperm malformation rate after thawing of Wandong cattle, and high sperm motility rate after thawing of Wannan cattle and Dabieshan cattle. |