湘西黄牛Y-SNPs遗传多样性研究

Genetic Diversity of Y-SNPs in Xiangxi Cattle

中文关键词: 湘西黄牛  Y-SNPs  单倍型组  父系起源

英文关键词: Xiangxi cattle  Y-SNPs  haplogroup  paternal origin

中文摘要:

      [目的]通过Y-SNP分子标记方法研究湘西黄牛的遗传多样性、群体遗传结构及父系起源。[方法]采用PCR扩增、测序与生物信息学方法，对24头湘西黄牛的2个Y-SNPs(UTY-19和ZFY-10)标记进行多态性分析。[结果]结果表明，湘西黄牛有Y1和Y3两种单倍型组，频率分别为12.5%和87.5%，表明湘西黄牛可能有普通牛和瘤牛2个父系起源。湘西黄牛的Y-SNP遗传多样度为0.2283±0.0978，表明湘西黄牛具有较低的父系遗传多样性，品种纯度较高。[结论]湘西黄牛的父系起源为瘤牛Y3单倍型组，其Y1单倍型组为国外肉牛杂交所致。

英文摘要:

      [Objective] To analyze the paternal origin, genetic diversity and population genetic structure of Xiangxi cattle, this study used Y-SNPs molecular markers. [Method] The authors Used PCR amplification, sequencing and bioinformatics methods, to carry out the polymorphism analysis of 24 Xiangxi cattle samples using two Y-SNPs markers (UTY-19 and ZFY-10). [Result] The results showed that there were two haplogroups of Y1 and Y3, with the frequencies of 12.5% and 87.5%, respectively, indicating that Xiangxi cattle might be two paternal origins of Bos taurus and Bos indicus. The genetic diversity of Y chromosome was 0.2283±0.0978, indicating that Xiangxi cattle had lower paternal genetic diversity and higher breed purity. [Conclusion] The paternal origin of Xiangxi cattle is zebu Y3 haplogroup, the Y1 haplogroup in Xiangxi cattle came from hybridization of foreign beef cattle breeds.