牛粪基质中添加不同发酵菌剂对蚯蚓生长繁殖的影响

Effects of Different Fermentation Agents on the Growth and Reproduction of Earthworms in Cow Manure Matrix

中文关键词: 牛粪  微生态发酵饲料  蚯蚓  生长繁殖

英文关键词: cow dung  microecological fermentation feed  earthworms  the growth and reproduction

中文摘要:

      [目的]对比分析各个发酵菌剂对蚯蚓生长繁殖性能的动态变化，根据蚯蚓生长状况，筛选出适合蚯蚓生长的最佳牛粪发酵菌，为牛粪发酵菌剂的开发研究提供参考。[方法]试验共计75 d，采用室内培养法，选取新鲜牛粪等分为4组，分别添加3种成品牛粪发酵添加菌剂，留一组空白对照，置于塑料培养盆中，25 ℃室温条件下预发酵15 d。接种蚯蚓消解牛粪间隔15 d分别破坏性取样，记录各物料组合中蚯蚓重、幼蚓数以及蚓茧数，对各物料组合中的蚯蚓生长、繁殖状况进行比较分析。[结果]研究结果表明，Ⅰ组牛粪中添加EM发酵菌剂、Ⅱ组添加中药微生态发酵菌剂、Ⅲ组添加生物发酵菌剂（nuclear）进行自然发酵，Ⅳ组空白对照为牛粪自然发酵，3种成品牛粪饲喂蚯蚓观察产生环带、产生蚓茧的时间，并都能促进蚯蚓的生长繁殖，蚯蚓个体重明显增高，Ⅱ组促进作用最明显，其次是Ⅰ组和Ⅲ组，Ⅳ组对照组最次。

英文摘要:

      [Objective] To compare and analyze the dynamic changes of the growth and reproduction performance of earthworms with different fermentative agents in cow manure matrix, and select the best fermentative bacteria for earthworm growth according to the growth condition of earthworm. The best bacteria suitable for the growth of earthworms were selected to provide reference for the development and research of the bacteria. [Method] The experiment was lasted 75 d. Fresh cow manure was selected and divided into four groups by indoor culture method. Three kinds of finished cow manure fermentation agents were added respectively, and a group of blank control was left, placed in a plastic culture pot, and pre-fermented for 15 d at 25 ℃ temperature. Earthworm inoculation cow dung destructive sampling interval of 15 d respectively, recorded the material composition of the earthworm weight, number of young earthworms and cocoon lumbrukinase, so as to compare the growth and reproduction of earthworms in different material combinations. [Result] The results showed that in group I, EM fermentation bacteria were added, group Ⅱ added Chinese medicine microecological fermentation bacteria, group Ⅲ added biological fermentation agent (nuclear) for natural fermentation, group Ⅳ blank control was cow manure natural fermentation. The three kinds of finished cow dung were fed to earthworms to observe the time of producing the loop and producing the worm cocoon, and can promote the growth of earthworms, earthworm weight all increased obviously in those three groups. Group Ⅱ had the most obvious promotion effect, followed by group I and group Ⅲ, and the control group Ⅳ had the least.