滇中牛Y-SNPs和Y-STRs遗传多样性研究

Genetic Diversity of Y-SNPs and Y-STRs in Dianzhong Cattle

中文关键词: 滇中牛  Y-SNPs  Y-STRs  遗传多样性  父系起源

英文关键词: Dianzhong cattle  Y-SNPs  Y-STRs  genetic diversity  paternal origin

中文摘要:

      [目的]为探究云南滇中牛的Y染色体遗传结构与血统来源，以期为该黄牛品种的资源保护与利用提供科学依据。[方法]采用PCR扩增和荧光分型方法，对27头滇中牛的Y-SNPs（UTY19和UTY10）和Y-STRs（INRA189和BM861）遗传多样性进行分析。[结果]27头滇中牛包含Y2和Y3两种单倍型组，其频率分别为22.22%和77.78%。结合Y-SNPs和Y-STRs的分型结果，发现这27头滇中牛存在Y2-90-158、Y3-88-156和Y3-90-156共3种Y染色体单倍型（Y-INRA189-BM861），Y染色体单倍型多样度为0.5128±0.0904，其遗传多样性较高。[结论]滇中牛Y染色体遗传多样性较高，有普通牛和瘤牛2个父系起源，以瘤牛血统为主。

英文摘要:

      [Objective] We explored the genetic structure and paternal origins of Y chromosome in Yunnan Dianzhong cattle to provide a scientific basis for the resource protection and utilization. [Methods] PCR amplification and fluorescence genotyping methods were used to detect the genetic diversity of Y-SNPs（UTY19 and ZFY10）and Y-STRs（INRA189 and BM861）in 27 Dianzhong cattle samples. [Results] All 27 Dianzhong samples belong to Y2 and Y3 haplogroup with frequencies of 22.22% and 77.78%, respectively. The Y chromosome haplotypes of Y2-90-158, Y3-88-156 and Y3-90-156 were identified in 27 Dianzhong cattle samples by combining Y-SNPs and Y-STRs genotyping data (Y-SNP-INRA189-BM861). The Y chromosome haplotype diversity was 0.5128±0.0904 indicating high paternal genetic diversity in Dianzhong cattle. [Conclusions] Dianzhong cattle has high paternal genetic diversity and two paternal origins of Bos taurus Y2 and Bos indicus Y3, of which, Bos indicus Y3 is obviously predominant paternal origin．